CIQTEK Cryo-SEM Solution Enables Damage-Free, High-Resolution Imaging of Biological Samples

In the fields of life sciences, biomedicine, food inspection, and soft matter research, achieving high-resolution imaging of hydrated and beam-sensitive samples has always been a major challenge. Conventional sample preparation methods, such as chemical fixation, dehydration, and drying, often result in shrinkage, deformation, or structural damage, leading to results that deviate from the true state of the sample.

Leveraging its advanced scanning electron microscopy technology, CIQTEK has introduced the Cryo-SEM Solution, which integrates low-temperature freezing and vacuum transfer. This enables in-situ, non-destructive, and high-fidelity microscopic observation of biological and sensitive samples, truly “freezing” the microscopic details of life.

With liquid-nitrogen slush rapid-freezing technology, samples can be vitrified instantly at -210 °C, preserving their original morphology and chemical composition to the greatest extent. The integrated cryo-preparation system combines freeze-fracture, sublimation coating, and low-temperature transfer, avoiding the complexity and potential errors of conventional manual preparation. Throughout the entire process, samples are maintained under cryogenic vacuum conditions and transferred to the SEM cryo-stage, where high-resolution imaging at -180 °C effectively suppresses electron beam damage and significantly improves image quality.

Cryo-prepared boxwood leaf showing intact vein structures, while the untreated sample exhibits severe shrinkage.

Yogurt

Mold

Cryo-prepared yogurt sample clearly reveals protein networks and fungal hyphae.

In addition, the system offers strong compatibility, adaptable across CIQTEK's full range of SEMs and dual-beam FIBSEM systems, meeting diverse needs from routine observation to advanced analysis.

The CIQTEK Cryo-SEM Solution is more than just a set of instruments. It embodies a scientific approach dedicated to faithfully restoring the microscopic world. It empowers researchers to overcome technical limitations, capture critical details at the micro scale of life, and drive both fundamental research and applied development to new heights.

September 26–30, Wuhan

At the 2025 Chinese National Electron Microscopy Academic Conference, CIQTEK will unveil eight cutting-edge EM solutions.

Stay tuned!